EBER Probe (In Situ Hybridization) , Automated

Description

This kit is used to detect E-B virus encoded RNA (EBER) by in situ hybridization (ISH) in formalin-fixed paraffin-embedded tissue sections, which can provide auxiliary information for the diagnosis in clinic.

1. Staining intensity

The nucleus of specific cells in the tissue section have yellow or brown-yellow coloration and no background coloration.

2. Sensitivity of probe

Test EBER positive tissues from lymphoma, stomach cancer, or nasopharyngeal cancer, the results are all positive.

3. Probe specificity

After the probe hybridized with HPV positive, EBER positive, and HBV positive tissues respectively, it is observed under ordinary light microscope that only EBER positive tissue samples show positive cell staining, the HPV and HBV positive samples show no positive staining.

4. In-batch repeatability

Stain sections from the same EBER tissue with the same batched reagent, with no significant difference in staining intensity or localization.

5. Batch repeatability

Staining of sections from the same EBER positive tissue with different batches of reagent does not differ significantly in the intensity or location of the staining.

Specifications

25 Tests/box, 50 Tests/box, 100 Tests/box.
Mainly including:
Digoxin labeled EBER probe, anti-digoxin antibody, pepsin working liquid.
Components not included but necessary for the test:
Immunochromogenic reagent; Washing solution: Washing Solution Concentrated (10×) is used for dilution.
Other reagents not provided:
EBER positive contrast photo (lymphoma); Xylene; Ethanol (anhydrous, 85%, 70%); Purified water; Neutral balsam; Rubber cement.