Immunohistochemical Secondary Antibody Detection Kit

Description

Micro Stacker is a self-developed non-biotin immunohistochemical detection system based on the core technology “micropolymer stacking method”.Staining on wax or frozen sections.

1. Improve sensitivity: For the first time, the micro-polymer skeleton structure is used, and the enzymes and antibodies are polymerized in layers, which makes the polymer structure more compact, the tissue penetration is stronger, and the sensitivity is increased by several times.
2. Improve specificity: The Fab’ antibody fragment labeling method is used for the first time to avoid background staining caused by the binding of the Fc segment in the intact antibody to the Fc receptor in the tissue; the non-biotin detection system avoids the occurrence of endogenous biotin in the tissue. background staining.
3. Improved stability: The new coupling chemistry is used for the first time, and the ratio of enzyme and antibody components is precisely controllable, making the staining results more stable and uniform.

Specifications

Manual secondary antibodies

Universal
1. It can be used for antibody detection of mouse and rabbit.
2. Can be widely used in immunohistochemistry of paraffin tissue sections and frozen sections
dyeing.
Recommended staining procedure (manual): primary antibody RT60min; secondary antibody RT30min; DAB5min
Enhanced
Features: On the basis of the universal type, the aggregation of enzymes and antibodies is further improved
The degree of integration, so the sensitivity is higher and the effect is equivalent to the airborne
Application:
1. It can be used for antibody detection of mouse and rabbit.
2. Can be widely used in immunohistochemistry of paraffin tissue sections and frozen sections
dyeing.
Element:
1. Peroxidase-conjugated anti-mouse/rabbit secondary antibody polymer
2. DAB substrate
3. DAB buffer
(optional: peroxidase blocking agent, hematoxylin)

Automated secondary antibodies

Flex Application:
1. It can be used for primary antibody detection of mouse and rabbit origin.
2. Suitable for various types of automatic immunohistochemical staining instruments.
Plus Application:
1. In the mouse/rabbit primary antibody project, the optimal effect can be achieved at the same time.
2. Applicable fast procedure (15min for primary antibody/8min after primary antibody/secondary antibody 8min/DAB10min).
Element
1. Peroxidase blocking agent
2. Primary antibody post-amplifier
3. Peroxidase-labeled secondary antibody polymer
4. DAB substrate
5. DAB buffer
6. Hematoxylin

Research secondary antibodies

Goat Anti-Rabbit Kit Application:
This product is mainly used for the staining of “rabbit monoclonal antibody/rabbit polyclonal antibody” on “mouse/rat” tissue.
Goat Anti-Mouse Kit
Application: This product is mainly used for the staining of “mouse monoclonal antibody” on “rabbit” tissue.
Rabbit-on-Rodent
Application: Mainly used for the staining of “rabbit monoclonal antibody/rabbit polyclonal antibody” on “mouse/rat” tissue.
Features: This product can significantly reduce non-specific staining caused by endogenous immunoglobulins through cross-adsorption of specific species of serum.
Element
1. Peroxidase-conjugated anti-mouse/rabbit secondary antibody polymer
2. DAB substrate
3. DAB buffer
(optional: peroxidase blocking agent, hematoxylin)

More Secondary Antibodies can be found in the PDF document