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Universal Polymer Staining Kit

  • Universal type
  • 20-1000 test/kit

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Description

In the immunohistochemical reaction, the target is bound to the primary antigen antibody and labeled by staining.
1.pH: the pH value of substrate buffer is 7.5~7.7.
2. Conformance: Use three positive tissues and corresponding antibody for test, using the same other related reagents, and the same immunohistochemical steps to stain different tissue section respectively, the results of positive tissue should be positive, located accurately, and no background staining, and blank control and negative control should be negative.
3. In-batch repeatability: Three tissue slices from the same tissue source containing the target antigen were immunohistochemical detected with the same batch number product, and there was no significant difference in the intensity and location of tissue slice staining.
4. Inter-batch repeatability: Immunohistochemical tests were performed on three tissue slices from the same tissue source containing the target antigen with three different batches of products, and there was no significant difference in the intensity and location of tissue slice staining.
5. Proper protective measures should be taken to avoiding contact with the skin and eyes and should not be inhaled into the mouth. If the reagents contact with the skin, mucosa and other parts of the body, a large amount of water should be used in time to wash the reagents.
6. During each staining, positive contrast slide and blank control must be used, otherwise the results cannot be used.
7. Improper antigen retrieval, incubation time, temperature conditions, or other application methods may lead to incorrect results.
8. When stored at room temperature, the samples should be stained within 7 days. Otherwise, the degeneration of the antigen in the tissue can produce false negative results.
9. If a positive control does not show an appropriate positive staining, indicates an operational error and the results of this batch of samples shall be invalid.
10.DAB has a potential mutagenic effect, Special attention should be paid to safety precautions during use. The storage and detoxication of waste liquid after use should also comply with relevant laws and regulations

Specifications

  1. It is mainly composed of endogenous peroxidase blocking reagent, enzyme-labeled goat anti-mouse/rabbit polymer, DAB substrate (20×), substrate buffer and hematoxylin.
  2. 20 test/kit, 100 test/kit, 200 test/kit, 300 test/kit, 1000 test/kit

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