An artificial environment is used for the placement of cells during cell development in cell culture. This makes sure that the requirement of oxygen along with other nutrients is supplied equally. It also ensures that the ideal temperature required for the development and growth of the cells is met.
An incubator along with a lab shaker is necessary for such circumstances to be met. The conditions of appropriate temperature are maintained by the incubator, whereas the combination and circulation of oxygen and different nutrients across the culture is done by the lab shaker.
As a solution to buying two different devices, a single device has been consolidated for the same purpose. This device is known by the name of an incubator shaker. Due to oxygen, temperature, and nutrients being delivered by a single system, the whole process of cell culturing is made more time effective. As a result, culture growth duration is reduced. Furthermore, because all of these factors are handled by a single apparatus, it makes it simpler to determine how different conditions influence cell development.
To opt for the best incubator shaker to buy, make sure that you are familiar with all the basic information regarding it. Read below to understand more:
All the information about A incubator shaker
When purchasing an incubator shaker to get the ideal cell culture outcome, it is important to consider the major characteristics and the function that each of these aspects plays.
Variable Speed of Rotation:The rotational speed of an incubator shaker is expressed in revolutions per minute (RPM). This metric indicates the number of times a fixed axis is rotated in a minute. The rotation speed changes with the type of cell. Plant cell types require between 100 and 150 revolutions per minute while animal cell types need between 40 and 100 revolutions per minute. As a result, ensure that your machine supports speed-based settings too.
Programmability:Programmability plays an important role. Memory should be present in the incubator shaker for time, speed, or temperature efficient based steps. This means that you should be able to write a series of steps for it to follow which helps in simplifying and speeding up the tests. For cell culture, qualities like these enable more exact control of factors that may either boost or retard it.
Temperature Control:This aspect is important as various cell types need different temperature settings to develop. The temperature conditions for different cells vary according to their type. For instance, mammalian cell development need a temperature of 37°C, amphibians of 15°C while plant cells should have the temperature between 20 and 30°C.
Sufficient Alarm System:An incubator shaker must be fitted with a sufficient alarm system. This system should provide both audible and visual alerts in the event of experimental failures because cell culturing is prone to mistakes like power error, open lid, etc. similar to any other laboratory experiment.
-Cell and tissue production in regenerative medicine
-Reproductive medicine controlled growth process in pharmaceutical research
-Cell cultrure ,tissue, grow bacteria and yeast, fungus, insect cells, plant cells and algae, plasmid dna production & protein expression, Seed cultures
-Safe tissue production with adherence to all international regulations and requirements such as cell culture under cleanroom conditions
-Food quality testing
-Studies of solubility
-It can cultivate microbial cells and various kinds of strains.
-Enzyme reactions- fermentation, hybridization, biochemistry and research of enzyme
Pay attention to the size
Most importantly when purchasing consider the size of the incubator. The size required is decided the effort required for a particular experiment and the size of your facility. There are various sizes available when it comes to floor-model, high capacity incubator shakers, and benchtop.
Large Capacity incubator shakers:
Comprehensive in terms of features, the big capacity has a stacking feature that expedites the culture process and expands laboratory capacity. These shakers are suggested for experiments with huge sample amounts to be processed as they require a large capacity to accommodate tubes and flasks of different sizes.
Floor- incubator shakers:
If the application of your cell culture demands a significant amount of work then you can opt for incubator shakers of floor-model if your laboratory has enough space. Additionally, it has strong workload shaking, as well as rapid cooling and heating performance. They are well-suited for high-volume applications, since they can carry six liter flasks which range up to five in number.
Benchtop incubator shakers:
Benchtop shakers fit into compact bench areas, making them portable and efficient. They are considered to be the smallest in size.
Types of Incubator Shakers
These shakers are controlled mechanically by dials and their adjustments are limited to laboratory mixing only. They come with limited options of settings and customizations.
Such units simplify the specification of attributes like time, velocity, and temperature. Some models have programming of multi-step too.
These maintain a specific amount of CO2 during the cell culture. Mammalian along with stem cells are two types of cell that have CO2 as their requirement for their flourishing.