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DNA/RNA Extraction Kit (Magnetic Beads), GFV502

  • Packaging: 96preps per box
  • The Virus DNA/RNA Kit (Magnetic Beads) is designed for rapid purification of high quality nucleic acid (RNA and DNA) from virus in samples such as swabs, saliva, blood, Bodily Fluid, Plasma/Serum, urine, and viral transport media (VTM).

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Description

Intended Use

The Virus DNA/RNA Kit (Magnetic Beads) is designed for rapid purification of high quality nucleic acid (RNA and DNA) from virus in samples such as swabs, saliva, blood, Bodily Fluid, Plasma/Serum, urine, and viral transport media (VTM).

Principle

The Virus DNA/RNA Kits (Magnetic Beads) are automation-ready plates prefilled suitably configured for NAE purification systems. Nucleic acids (DNA/RNA) from a complex with magnetic beads in a specially formulated buffer. The beads / nucleic acids complex is then separated from lysates using a magnet. Purified DNA/RNA are then eluted when the buffer condition is adjusted. Special magnetic beads technology enables purification of high-quality nucleic acids that are free of proteins, nucleases, and other impurities.

Packaging

96preps per box

Work Station

NAE-0132/NAE-0196

Storage

All Reagents can be stored at room temperature (15–25°C) for 12 months.

Protocol

  1. Take out prefilled 96-well plates from the box, gently upside down to mix the beads. Flick downward or gently tap each plate before removing the
  2. Add 200μl of sample to each well of Plate1(MVN).

Note: The sample needs to be equilibrated to room temperature.

  1. Put the 96 Tip Comb into the Plate2 (DW1P), slot into deck position 2 on the Purifier
  2. Immediately load the remaining plates onto the instrument as
  3. Select the program “GF_FM502T5_P96” and start the
  4. Atthe end of the run, immediately remove the Plate4 (ddH2O) from the instrument, then transfer the solution to the final tubes/plate and

Note: The purified nucleic acid is ready for immediate use. Alternatively, store the plate at –20°C for long term storage.

Precautions

  1. Always wear a suitable lab coat, disposable gloves, and protective
  2. Precipitatesand high viscosity can occur if plates or solutions are stored in a refrigerator or when the room temperature is too cold. If there are precipitates in these solutions, warm them at 37°C and gently mix to dissolve precipitates. Avoid creating
  3. Yellowing of the Lysis/Binding and Washing Solution is normal and will not impact buffer performance.

Symbols

Symbols Meanings
 DNA Extraction Kit Symbols  

Manufacturer

DNA Extraction Kit Symbols  Authorized representative in the European Community
DNA Extraction Kit Symbols  In Vitro Diagnostic Medical Device
DNA Extraction Kit Symbols This product fulfills the requirements of the European Directive 98/79 EC for in vitro diagnostic medical devices.

 

Specifications

Table1. Kit Contents

Contents Units
Plate 1 Buffer MVN 500μL/well
Plate 2 Buffer DW1P 500μL/well
 

Plate 3

Buffer MWP 600μL/well
FineMag Particles G 10μL/well
Plate 4 RNase-Free ddH2O 100μL/well
96 Tip Comb 1

 

Table2. Nucleic acid purification procedure

 

Step

 

Posi- tion

 

Name

Agitation  

Volume

(ul)

Heat Magnetization Out of tube
Amplitude Frequency Time

(s)

Temperature Time

(s)

Time

(s)

Time

(s)

1 2 load
2 3 binding low fastest 30 600 20sec,Loop 2
3 1 binding low fastest 360 700 45-mix 360 30sec,Loop 2
4 2 washing low fastest 100 500 20sec,Loop 2
5 3 washing low fastest 60 600 20sec,Loop 2 120
6 4 elution low middle 240 100 70-mix 240 15sec,Loop 3
7 2 unload

Title

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    Contact Info

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    Phone: +86-531-88982330

    Fax: +86-531-88983691

    Web: www.bioevopeak.com

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